AustralieFrV1, Main, Exploration, bibRecord, 008D22

Functional coupling of μ-receptor-Gαi-tethered proteins in AtT20 cells

Identifieur interne : 008D22 ( Main/Exploration ); précédent : 008D21; suivant : 008D23

Functional coupling of μ-receptor-Gαi-tethered proteins in AtT20 cells

Auteurs : Billy C. Chieng [Australie] ; David J. Lee [Australie] ; Yan P. Du [Australie] ; Peregrine B. Osborne [Australie] ; Macdonald J. Christie [Australie] ; Dominique Massotte [France]

Source :

Neuroreport : (Oxford) [ 0959-4965 ] ; 2008.

RBID : Pascal:09-0026231

Descripteurs français

Pascal (Inist)
- Récepteur biologique, Protéine G, Courant ionique, Calcium, Opiacés, Toxine, Potassium, Peptide opioïde.
Wicri :
- topic : Calcium, Potassium.

English descriptors

KwdEn :
- Biological receptor, Calcium, G protein, Ionic current, Opiates, Opioid peptide, Potassium, Toxin.

Abstract

Opioid efficacy on μ-receptor may be influenced by various G_i/o-G-protein subunits interacting with intracellular face of receptor. Pertussis toxin-insensitive Gα_il and Gα_i2 subunits tethered with μ-receptor were stably transfected into AtT20 cells to (i) determine coupling of different α-subunits on opioid efficacy, and (ii) determine coupling to downstream effectors, for example, calcium and potassium channels. After pertussis toxin, stimulation of [³⁵S]GTP-γ-S incorporation persisted. Both constructs were able to couple to native calcium and potassium channels, with endomorphins I and 2 equally effective. However, pertussis toxin abolished opioid actions on calcium and potassium channels suggesting strong coupling to endogenous G-proteins, and that differences in coupling efficacy to Gα_j1 and Gα_j2 previously observed are restricted to initial step of signaling cascade.

Affiliations:

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 003086
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to stream Main, to step Curation: 008D22

Le document en format XML

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<front><div type="abstract" xml:lang="en">Opioid efficacy on μ-receptor may be influenced by various G<sub>i/o</sub>
-G-protein subunits interacting with intracellular face of receptor. Pertussis toxin-insensitive Gα<sub>il</sub>
 and Gα<sub>i2</sub>
 subunits tethered with μ-receptor were stably transfected into AtT20 cells to (i) determine coupling of different α-subunits on opioid efficacy, and (ii) determine coupling to downstream effectors, for example, calcium and potassium channels. After pertussis toxin, stimulation of [<sup>35</sup>
S]GTP-γ-S incorporation persisted. Both constructs were able to couple to native calcium and potassium channels, with endomorphins I and 2 equally effective. However, pertussis toxin abolished opioid actions on calcium and potassium channels suggesting strong coupling to endogenous G-proteins, and that differences in coupling efficacy to Gα<sub>j1</sub>
 and Gα<sub>j2</sub>
 previously observed are restricted to initial step of signaling cascade.</div>
</front>
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<name sortKey="Christie, Macdonald J" sort="Christie, Macdonald J" uniqKey="Christie M" first="Macdonald J." last="Christie">Macdonald J. Christie</name>
<name sortKey="Du, Yan P" sort="Du, Yan P" uniqKey="Du Y" first="Yan P." last="Du">Yan P. Du</name>
<name sortKey="Lee, David J" sort="Lee, David J" uniqKey="Lee D" first="David J." last="Lee">David J. Lee</name>
<name sortKey="Osborne, Peregrine B" sort="Osborne, Peregrine B" uniqKey="Osborne P" first="Peregrine B." last="Osborne">Peregrine B. Osborne</name>
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<country name="France"><noRegion><name sortKey="Massotte, Dominique" sort="Massotte, Dominique" uniqKey="Massotte D" first="Dominique" last="Massotte">Dominique Massotte</name>
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Functional coupling of μ-receptor-Gαi-tethered proteins in AtT20 cells

Functional coupling of μ-receptor-Gαi-tethered proteins in AtT20 cells

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri